ICSE – Grade 9 – Biology – Ch 09 – QA

Questions & Answers

ICSE - Grade - 9

Subject: Biology

Chapter - 09 - Economic Importance of Bacteria and Fungi

Types of Questions

MCQ

  1. Which group of organisms are considered prokaryotic?
     a) Algae
     b) Fungi
     c) Bacteria
     d) Protozoa
     Answer: c) Bacteria


  2. Which of the following is used in the production of antibiotics?
     a) Yeast
     b) Penicillium
     c) Mushroom
     d) Nostoc
     Answer: b) Penicillium


  3. Which vaccine is prepared by using weakened or killed bacteria?
     a) Toxoid
     b) Serum
     c) Vaccine
     d) Antibiotic
     Answer: c) Vaccine


  4. Which of the following destroys bacterial toxins inside the body?
     a) Antitoxin
     b) Vaccine
     c) Serum
     d) Antibiotic
     Answer: a) Antitoxin


  5. Which type of bacteria convert atmospheric nitrogen into compounds usable by plants?
     a) Denitrifying bacteria
     b) Nitrifying bacteria
     c) Nitrogen-fixing bacteria
     d) Saprophytic bacteria
     Answer: c) Nitrogen-fixing bacteria


  6. Rhizobium is an example of:
     a) Nitrifying bacteria
     b) Nitrogen-fixing bacteria
     c) Denitrifying bacteria
     d) Pathogenic bacteria
     Answer: b) Nitrogen-fixing bacteria


  7. Which bacteria convert ammonia into nitrates?
     a) Denitrifying bacteria
     b) Saprophytes
     c) Nitrifying bacteria
     d) Nitrogen-fixing bacteria
     Answer: c) Nitrifying bacteria


  8. Which bacteria convert nitrates into free nitrogen?
     a) Nitrogen-fixing
     b) Nitrifying
     c) Denitrifying
     d) Saprophytic
     Answer: c) Denitrifying


  9. Which of the following bacteria is involved in the production of curd?
     a) Rhizobium
     b) Lactobacillus
     c) Salmonella
     d) Clostridium
     Answer: b) Lactobacillus


  10. Which process spoils food by bacteria?
     a) Photosynthesis
     b) Putrefaction
     c) Fermentation
     d) Nitrogen fixation
     Answer: b) Putrefaction


  11. Which of the following is a preventive measure against bacterial food spoilage?
     a) Freezing
     b) Sun drying
     c) Salting
     d) All of these
     Answer: d) All of these


  12. Which of the following is a bacterial disease in plants?
     a) Citrus canker
     b) Smut
     c) Rust
     d) Wilt of cotton
     Answer: a) Citrus canker


  13. Which of the following is a bacterial disease in animals?
     a) Foot and mouth disease
     b) Tuberculosis
     c) Ringworm
     d) Rust
     Answer: b) Tuberculosis


  14. The bacterium used in leather tanning is:
     a) Lactobacillus
     b) Rhizobium
     c) Clostridium
     d) Streptococcus
     Answer: c) Clostridium


  15. Antibiotics are obtained from:
     a) Bacteria and fungi
     b) Algae only
     c) Viruses only
     d) Protozoa
     Answer: a) Bacteria and fungi


  16. Which of the following is the first discovered antibiotic?
     a) Streptomycin
     b) Penicillin
     c) Tetracycline
     d) Chloramphenicol
     Answer: b) Penicillin


  17. Serums provide:
     a) Natural immunity
     b) Active immunity
     c) Passive immunity
     d) Artificial immunity
     Answer: c) Passive immunity


  18. Vaccines provide:
     a) Passive immunity
     b) Natural immunity
     c) Active immunity
     d) Temporary immunity
     Answer: c) Active immunity


  19. Toxoids are:
     a) Weakened pathogens
     b) Modified toxins
     c) Antibodies
     d) Antibiotics
     Answer: b) Modified toxins


  20. Which of the following gives flavour to tea during fermentation?
     a) Bacteria
     b) Viruses
     c) Algae
     d) Protozoa
     Answer: a) Bacteria


  21. Which microorganism is used in brewing industry?
     a) Penicillium
     b) Rhizopus
     c) Saccharomyces
     d) Clostridium
     Answer: c) Saccharomyces


  22. Which process is carried out by yeast in wine production?
     a) Photosynthesis
     b) Putrefaction
     c) Alcoholic fermentation
     d) Nitrification
     Answer: c) Alcoholic fermentation


  23. In bread-making, yeast produces:
     a) Oxygen and alcohol
     b) Carbon dioxide and alcohol
     c) Ammonia and nitrogen
     d) Acids
     Answer: b) Carbon dioxide and alcohol


  24. Which of the following fungi is edible?
     a) Penicillium
     b) Agaricus
     c) Rhizopus
     d) Aspergillus
     Answer: b) Agaricus


  25. Which of the following increases the softness and porosity of bread?
     a) Alcohol
     b) Carbon dioxide
     c) Oxygen
     d) Enzymes
     Answer: b) Carbon dioxide


  26. Cheese is produced by:
     a) Algae
     b) Bacteria
     c) Yeast
     d) Virus
     Answer: b) Bacteria


  27. Which fungus is used in the production of penicillin?
     a) Penicillium notatum
     b) Saccharomyces cerevisiae
     c) Rhizopus stolonifer
     d) Aspergillus niger
     Answer: a) Penicillium notatum


  28. Mushroom is rich in:
     a) Carbohydrates only
     b) Proteins and vitamins
     c) Fats only
     d) Starch
     Answer: b) Proteins and vitamins


  29. The nutritive value of mushrooms is closest to:
     a) Meat
     b) Fruits
     c) Cereals
     d) Leafy vegetables
     Answer: a) Meat


  30. Which fungus is used in the production of citric acid?
     a) Aspergillus niger
     b) Saccharomyces
     c) Rhizopus
     d) Mucor
     Answer: a) Aspergillus niger


  31. Spoilage of bread is caused by:
     a) Yeast
     b) Penicillium
     c) Rhizopus
     d) Both b and c
     Answer: d) Both b and c


  32. Which bacterial process reduces soil fertility?
     a) Nitrogen fixation
     b) Nitrification
     c) Denitrification
     d) None of these
     Answer: c) Denitrification


  33. Antibiotic “Streptomycin” is obtained from:
     a) Fungi
     b) Bacteria
     c) Virus
     d) Protozoa
     Answer: b) Bacteria


  34. Which disease is caused by Mycobacterium tuberculosis?
     a) Anthrax
     b) Typhoid
     c) Tuberculosis
     d) Plague
     Answer: c) Tuberculosis


  35. Which fungus is commonly called baker’s yeast?
     a) Saccharomyces cerevisiae
     b) Aspergillus niger
     c) Rhizopus stolonifer
     d) Penicillium
     Answer: a) Saccharomyces cerevisiae


  36. Which of the following is a harmful role of bacteria?
     a) Nitrification
     b) Nitrogen fixation
     c) Food spoilage
     d) Leather tanning
     Answer: c) Food spoilage


  37. Antibiotics act against:
     a) Viruses only
     b) Bacteria only
     c) Both bacteria and viruses
     d) Protozoa
     Answer: b) Bacteria only


  38. Clostridium tetani causes:
     a) Tuberculosis
     b) Typhoid
     c) Tetanus
     d) Anthrax
     Answer: c) Tetanus


  39. Yeast reproduces mainly by:
     a) Binary fission
     b) Budding
     c) Fragmentation
     d) Spore formation
     Answer: b) Budding


  40. Which of the following is not a fungal product?
     a) Wine
     b) Bread
     c) Cheese
     d) Serum
     Answer: d) Serum


  41. Which of the following is not an antibiotic?
     a) Penicillin
     b) Streptomycin
     c) Tetracycline
     d) Vaccine
     Answer: d) Vaccine


  42. Which fungus is used in the ripening of cheese?
     a) Penicillium
     b) Rhizopus
     c) Aspergillus
     d) Yeast
     Answer: a) Penicillium


  43. Which bacterium is used in sewage treatment?
     a) Rhizobium
     b) Nitrosomonas
     c) Pseudomonas
     d) Clostridium
     Answer: c) Pseudomonas


  44. Who discovered Penicillin?
     a) Robert Koch
     b) Louis Pasteur
     c) Alexander Fleming
     d) Edward Jenner
     Answer: c) Alexander Fleming


  45. Which fungus is commonly called black bread mould?
     a) Penicillium
     b) Rhizopus
     c) Aspergillus
     d) Yeast
     Answer: b) Rhizopus


  46. A toxoid is used to immunize against:
     a) Viral diseases
     b) Bacterial toxins
     c) Fungal infection
     d) Food poisoning
     Answer: b) Bacterial toxins


  47. Which of the following methods preserves milk?
     a) Pasteurisation
     b) Fermentation
     c) Putrefaction
     d) Distillation
     Answer: a) Pasteurisation


  48. Which is a fungal disease in humans?
     a) Ringworm
     b) Typhoid
     c) Tetanus
     d) Cholera
     Answer: a) Ringworm


  49. Which fungus is edible and cultivated commercially?
     a) Agaricus
     b) Penicillium
     c) Aspergillus
     d) Rhizopus
     Answer: a) Agaricus


  50. Which of the following is both useful and harmful?
     a) Yeast
     b) Bacteria
     c) Fungi
     d) All of these
     Answer: d) All of these

Fill in the Blanks

  1. Bacteria are ______ organisms without a true nucleus.
     Answer: prokaryotic


  2. The bacterium Rhizobium is found in the root nodules of ______ plants.
     Answer: leguminous


  3. Bacteria like Rhizobium help in ______ fixation.
     Answer: nitrogen


  4. Conversion of ammonia into nitrites and nitrates is called ______.
     Answer: nitrification


  5. Conversion of nitrates into free nitrogen is called ______.
     Answer: denitrification


  6. ______ bacteria reduce soil fertility by releasing nitrogen gas.
     Answer: Denitrifying


  7. Lactobacillus bacteria convert milk sugar into ______ during curd formation.
     Answer: lactic acid


  8. The bacterium Clostridium is used in ______ tanning.
     Answer: leather


  9. Food spoilage by bacteria is mainly due to the process of ______.
     Answer: putrefaction


  10. Preservation of food by heating and cooling milk is called ______.
     Answer: pasteurisation


  11. Citrus canker is a ______ disease in plants.
     Answer: bacterial


  12. Tuberculosis is a ______ disease in humans.
     Answer: bacterial


  13. Antibiotics are obtained from both ______ and ______.
     Answer: bacteria, fungi


  14. The first discovered antibiotic was ______.
     Answer: Penicillin


  15. Penicillin was discovered by ______.
     Answer: Alexander Fleming


  16. Streptomycin is obtained from ______ bacteria.
     Answer: Streptomyces


  17. ______ provide active immunity to the body.
     Answer: Vaccines


  18. ______ provide passive immunity to the body.
     Answer: Serums


  19. ______ neutralise toxins produced by bacteria.
     Answer: Antitoxins


  20. ______ are modified toxins used for vaccination.
     Answer: Toxoids


  21. The flavour of tea is enhanced by the action of ______.
     Answer: bacteria


  22. Yeast belongs to the group of organisms called ______.
     Answer: fungi


  23. Yeast respires anaerobically to produce ______ and ______.
     Answer: alcohol, carbon dioxide


  24. In bread making, the ______ gas released by yeast makes the dough soft and porous.
     Answer: carbon dioxide


  25. The fungus used in brewing and baking is ______.
     Answer: Saccharomyces cerevisiae


  26. The fungus Penicillium notatum is used in producing the antibiotic ______.
     Answer: Penicillin


  27. Mushrooms such as ______ are edible and cultivated commercially.
     Answer: Agaricus


  28. Mushrooms are rich in ______ and vitamins.
     Answer: proteins


  29. The nutritive value of mushrooms is closest to that of ______.
     Answer: meat


  30. Citric acid is produced with the help of the fungus ______.
     Answer: Aspergillus niger


  31. Cheese is prepared with the help of ______.
     Answer: bacteria


  32. Some varieties of cheese are ripened with ______ fungi.
     Answer: Penicillium


  33. The fungus commonly called “black bread mould” is ______.
     Answer: Rhizopus


  34. Ringworm in humans is caused by ______ infection.
     Answer: fungal


  35. ______ is a preventive measure against bacterial food spoilage by reducing water content.
     Answer: Sun drying


  36. ______ is a preventive measure against bacterial food spoilage by low temperature.
     Answer: Freezing


  37. The vaccine for smallpox was discovered by ______.
     Answer: Edward Jenner


  38. ______ is a bacterial disease in cattle.
     Answer: Anthrax


  39. In alcoholic fermentation, sugar is converted into ______ and alcohol.
     Answer: carbon dioxide


  40. Pseudomonas bacteria are used in ______ treatment.
     Answer: sewage


  41. ______ is a fungal product used in making bread and alcoholic drinks.
     Answer: Yeast


  42. The bacteria that cause tetanus are called ______.
     Answer: Clostridium tetani


  43. A serum contains ready-made ______ to fight diseases.
     Answer: antibodies


  44. The antibiotic “tetracycline” is produced by ______.
     Answer: Streptomyces


  45. ______ discovered the process of pasteurisation.
     Answer: Louis Pasteur


  46. ______ are unicellular fungi used in industries.
     Answer: Yeasts


  47. The fungus used in commercial production of enzymes and organic acids is ______.
     Answer: Aspergillus


  48. The bacterial disease of rice caused by Xanthomonas oryzae is ______.
     Answer: bacterial blight


  49. The edible mushroom is ______, while poisonous mushrooms include ______.
     Answer: Agaricus, Amanita


  50. Antibiotics are effective against ______ but not against ______.
     Answer: bacteria, viruses

Name the Following

  1. Name the bacteria that fix atmospheric nitrogen in root nodules of legumes.
     Answer: Rhizobium


  2. Name the bacteria that convert ammonia into nitrites.
     Answer: Nitrosomonas


  3. Name the bacteria that convert nitrites into nitrates.
     Answer: Nitrobacter


  4. Name the bacteria that convert nitrates into free nitrogen.
     Answer: Pseudomonas denitrificans


  5. Name the bacteria used in the production of curd.
     Answer: Lactobacillus


  6. Name the bacterium used in leather tanning.
     Answer: Clostridium


  7. Name one bacterial disease of plants.
     Answer: Citrus canker


  8. Name one bacterial disease of animals.
     Answer: Anthrax


  9. Name one bacterial disease of humans.
     Answer: Tuberculosis


  10. Name the bacterium that causes tetanus.
     Answer: Clostridium tetani


  11. Name the bacterium that causes typhoid.
     Answer: Salmonella typhi


  12. Name the first discovered antibiotic.
     Answer: Penicillin


  13. Name the fungus used in the production of Penicillin.
     Answer: Penicillium notatum


  14. Name the scientist who discovered Penicillin.
     Answer: Alexander Fleming


  15. Name one antibiotic obtained from bacteria.
     Answer: Streptomycin


  16. Name the scientist who discovered pasteurisation.
     Answer: Louis Pasteur


  17. Name the scientist who discovered the smallpox vaccine.
     Answer: Edward Jenner


  18. Name the type of immunity given by vaccines.
     Answer: Active immunity


  19. Name the type of immunity given by serums.
     Answer: Passive immunity


  20. Name the substances that neutralise toxins of bacteria.
     Answer: Antitoxins


  21. Name the modified toxins used for vaccination.
     Answer: Toxoids


  22. Name the process by which milk is preserved by heating and cooling.
     Answer: Pasteurisation


  23. Name the process by which bacteria spoil food.
     Answer: Putrefaction


  24. Name one preventive method to stop bacterial spoilage of food.
     Answer: Freezing (or Salting / Sun drying)


  25. Name the bacterium used in sewage treatment.
     Answer: Pseudomonas


  26. Name the fungus used in brewing and baking industries.
     Answer: Saccharomyces cerevisiae


  27. Name the common name of Saccharomyces cerevisiae.
     Answer: Baker’s yeast


  28. Name the gas produced by yeast during bread-making.
     Answer: Carbon dioxide


  29. Name the alcoholic beverage prepared with yeast fermentation.
     Answer: Wine


  30. Name one fungus used in cheese ripening.
     Answer: Penicillium


  31. Name the edible mushroom commonly cultivated.
     Answer: Agaricus


  32. Name one poisonous mushroom.
     Answer: Amanita


  33. Name one fungus that causes spoilage of bread.
     Answer: Rhizopus


  34. Name the common name of Rhizopus.
     Answer: Black bread mould


  35. Name one fungus used in the production of citric acid.
     Answer: Aspergillus niger


  36. Name one fungal disease in humans.
     Answer: Ringworm


  37. Name the organism used in the production of alcohol.
     Answer: Yeast


  38. Name the organism used in the production of vinegar.
     Answer: Acetobacter


  39. Name the bacteria that give flavour to tea.
     Answer: Bacteria (specific fermenting bacteria)


  40. Name the bacterium used in the production of antibiotics.
     Answer: Streptomyces


  41. Name the antibiotic obtained from Streptomyces.
     Answer: Streptomycin


  42. Name the first vaccine discovered in history.
     Answer: Smallpox vaccine


  43. Name the edible part of a mushroom.
     Answer: Fruiting body


  44. Name the fungus that reproduces mainly by budding.
     Answer: Yeast


  45. Name the type of sugar fermented by yeast.
     Answer: Glucose


  46. Name one fungus that produces organic acids commercially.
     Answer: Aspergillus


  47. Name the bacteria that provide fertility to soil.
     Answer: Nitrogen-fixing bacteria


  48. Name the bacteria that reduce soil fertility.
     Answer: Denitrifying bacteria


  49. Name the industry where Clostridium is used.
     Answer: Leather tanning industry


  50. Name the industry where Saccharomyces is used.
     Answer: Brewing and baking industry

Answer in One Word

  1. The bacteria that fix atmospheric nitrogen in legumes.
     Answer: Rhizobium


  2. The process of conversion of ammonia into nitrites and nitrates.
     Answer: Nitrification


  3. The bacteria that convert nitrates into free nitrogen.
     Answer: Denitrifying bacteria


  4. The bacterium that converts milk to curd.
     Answer: Lactobacillus


  5. The bacterium used in leather tanning.
     Answer: Clostridium


  6. The disease of plants caused by bacteria.
     Answer: Citrus canker


  7. The bacterial disease of cattle.
     Answer: Anthrax


  8. The bacterial disease of humans caused by Mycobacterium tuberculosis.
     Answer: Tuberculosis


  9. The bacterium causing typhoid.
     Answer: Salmonella typhi


  10. The bacterium causing tetanus.
     Answer: Clostridium tetani


  11. The first discovered antibiotic.
     Answer: Penicillin


  12. The fungus used to produce penicillin.
     Answer: Penicillium


  13. The scientist who discovered penicillin.
     Answer: Alexander Fleming


  14. An antibiotic obtained from Streptomyces.
     Answer: Streptomycin


  15. The scientist who discovered pasteurisation.
     Answer: Louis Pasteur


  16. The scientist who discovered the smallpox vaccine.
     Answer: Edward Jenner


  17. The immunity developed by vaccines.
     Answer: Active


  18. The immunity developed by serums.
     Answer: Passive


  19. The substances that neutralise toxins of bacteria.
     Answer: Antitoxins


  20. Modified toxins used as vaccines.
     Answer: Toxoids


  21. The process of heating milk to destroy bacteria.
     Answer: Pasteurisation


  22. The process by which bacteria spoil food.
     Answer: Putrefaction


  23. A method of food preservation by drying.
     Answer: Dehydration


  24. A bacterium used in sewage treatment.
     Answer: Pseudomonas


  25. The unicellular fungus used in baking.
     Answer: Yeast


  26. The common name of Saccharomyces cerevisiae.
     Answer: Baker’s yeast


  27. The gas responsible for the porous texture of bread.
     Answer: Carbon dioxide


  28. The beverage obtained by fermentation of sugar by yeast.
     Answer: Wine


  29. The fungus used in cheese ripening.
     Answer: Penicillium


  30. An edible mushroom.
     Answer: Agaricus


  31. A poisonous mushroom.
     Answer: Amanita


  32. The fungus commonly called black bread mould.
     Answer: Rhizopus


  33. A fungal disease in humans.
     Answer: Ringworm


  34. The acid produced by Aspergillus niger.
     Answer: Citric


  35. The bacterium that adds fertility to the soil.
     Answer: Nitrogen-fixing


  36. The bacteria that lower soil fertility.
     Answer: Denitrifying


  37. A bacterial disease transmitted through contaminated water.
     Answer: Cholera


  38. A fungal product used in baking and brewing.
     Answer: Yeast


  39. The antibiotic obtained from Penicillium notatum.
     Answer: Penicillin


  40. The edible part of a mushroom.
     Answer: Fruiting body


  41. The process by which yeast reproduces.
     Answer: Budding


  42. The sugar fermented by yeast.
     Answer: Glucose


  43. The fungus used in commercial enzyme production.
     Answer: Aspergillus


  44. The bacterium that causes bacterial blight of rice.
     Answer: Xanthomonas oryzae


  45. The antibiotic discovered by Selman Waksman.
     Answer: Streptomycin


  46. The disease prevented by BCG vaccine.
     Answer: Tuberculosis


  47. The product formed when yeast ferments sugar anaerobically.
     Answer: Alcohol


  48. The microorganism that gives flavour to tea.
     Answer: Bacteria


  49. The organism that converts alcohol into vinegar.
     Answer: Acetobacter


  50. The fungus cultivated as food and rich in proteins.
     Answer: Mushroom

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Find the Odd Man Out

  1. Rhizobium, Nitrosomonas, Nitrobacter, Salmonella
     Answer: Salmonella
     Explanation: Salmonella is a pathogenic bacterium, others are useful in nitrogen cycle.


  2. Lactobacillus, Clostridium, Penicillium, Rhizobium
     Answer: Penicillium
     Explanation: Penicillium is a fungus, others are bacteria.


  3. Typhoid, Cholera, Tuberculosis, Ringworm
     Answer: Ringworm
     Explanation: Ringworm is a fungal disease, others are bacterial diseases.


  4. Citrus canker, Anthrax, Rust of wheat, Tuberculosis
     Answer: Rust of wheat
     Explanation: Rust of wheat is a fungal disease, others are bacterial diseases.


  5. Penicillin, Streptomycin, Vaccine, Tetracycline
     Answer: Vaccine
     Explanation: Vaccine gives immunity, others are antibiotics.


  6. Antitoxin, Toxoid, Serum, Penicillin
     Answer: Penicillin
     Explanation: Penicillin is an antibiotic, others are related to immunity.


  7. Nitrification, Denitrification, Putrefaction, Photosynthesis
     Answer: Photosynthesis
     Explanation: Photosynthesis is carried out by plants, others are bacterial processes.


  8. Freezing, Salting, Sun drying, Fermentation
     Answer: Fermentation
     Explanation: Fermentation spoils food, others preserve it.


  9. Penicillium, Aspergillus, Rhizopus, Lactobacillus
     Answer: Lactobacillus
     Explanation: Lactobacillus is a bacterium, others are fungi.


  10. Alexander Fleming, Edward Jenner, Louis Pasteur, Gregor Mendel
     Answer: Gregor Mendel
     Explanation: Mendel is the father of genetics, others worked on microbes/vaccines.


  11. Curd, Cheese, Vinegar, Tuberculosis
     Answer: Tuberculosis
     Explanation: Tuberculosis is a disease, others are microbial products.


  12. Bread, Wine, Beer, Anthrax
     Answer: Anthrax
     Explanation: Anthrax is a bacterial disease, others are products of fermentation.


  13. Agaricus, Amanita, Penicillium, Lactobacillus
     Answer: Lactobacillus
     Explanation: Lactobacillus is a bacterium, others are fungi.


  14. Carbon dioxide, Alcohol, Oxygen, Lactic acid
     Answer: Oxygen
     Explanation: Yeast does not produce oxygen during fermentation, others are products.


  15. Nitrosomonas, Nitrobacter, Rhizobium, Plasmodium
     Answer: Plasmodium
     Explanation: Plasmodium is a protozoan parasite, others are bacteria.


  16. Cheese, Bread, Wine, Cholera
     Answer: Cholera
     Explanation: Cholera is a disease, others are useful products of microbes.


  17. Clostridium tetani, Salmonella typhi, Mycobacterium tuberculosis, Aspergillus niger
     Answer: Aspergillus niger
     Explanation: Aspergillus is a fungus, others are bacteria.


  18. Vaccine, Serum, Antitoxin, Nitrification
     Answer: Nitrification
     Explanation: Nitrification is a soil bacterial process, others are immunity-related.


  19. Typhoid, Tetanus, Tuberculosis, Smallpox
     Answer: Smallpox
     Explanation: Smallpox is viral, others are bacterial diseases.


  20. Citric acid, Acetic acid, Lactic acid, Penicillin
     Answer: Penicillin
     Explanation: Penicillin is an antibiotic, others are microbial acids.


  21. Freezing, Boiling, Salting, Smoking
     Answer: Boiling
     Explanation: Boiling is a temporary method, others preserve food for longer periods.


  22. Agaricus, Yeast, Amanita, Anthrax
     Answer: Anthrax
     Explanation: Anthrax is a bacterial disease, others are fungi.


  23. Streptomycin, Tetracycline, Chloramphenicol, Vaccine
     Answer: Vaccine
     Explanation: Vaccine is not an antibiotic, others are antibiotics.


  24. Wine, Beer, Bread, Ringworm
     Answer: Ringworm
     Explanation: Ringworm is a fungal disease, others are products of yeast.


  25. Rhizopus, Penicillium, Aspergillus, Salmonella
     Answer: Salmonella
     Explanation: Salmonella is a bacterium, others are fungi.


  26. Putrefaction, Fermentation, Pasteurisation, Curdling
     Answer: Pasteurisation
     Explanation: Pasteurisation preserves food, others are microbial processes.


  27. Anthrax, Citrus canker, Tuberculosis, Malaria
     Answer: Malaria
     Explanation: Malaria is protozoan, others are bacterial diseases.


  28. Agaricus, Amanita, Lactobacillus, Penicillium
     Answer: Lactobacillus
     Explanation: Lactobacillus is a bacterium, others are fungi.


  29. Edward Jenner, Alexander Fleming, Louis Pasteur, Charles Darwin
     Answer: Charles Darwin
     Explanation: Darwin worked on evolution, others on microbes and vaccines.


  30. Penicillin, Cheese, Wine, Anthrax
     Answer: Anthrax
     Explanation: Anthrax is a disease, others are useful microbial products.


  31. Ringworm, Rust, Smut, Tuberculosis
     Answer: Tuberculosis
     Explanation: Tuberculosis is bacterial, others are fungal diseases.


  32. Pasteurisation, Freezing, Sun drying, Fermentation
     Answer: Fermentation
     Explanation: Fermentation spoils food, others preserve food.


  33. Rhizobium, Nitrosomonas, Nitrobacter, Aspergillus
     Answer: Aspergillus
     Explanation: Aspergillus is a fungus, others are bacteria in nitrogen cycle.


  34. Acetobacter, Penicillium, Aspergillus, Rhizopus
     Answer: Acetobacter
     Explanation: Acetobacter is a bacterium, others are fungi.


  35. Anthrax, Tuberculosis, Tetanus, Ringworm
     Answer: Ringworm
     Explanation: Ringworm is fungal, others are bacterial diseases.


  36. Wine, Bread, Beer, Tuberculosis
     Answer: Tuberculosis
     Explanation: Tuberculosis is a disease, others are useful products of yeast.


  37. Nitrification, Denitrification, Nitrogen fixation, Photosynthesis
     Answer: Photosynthesis
     Explanation: Photosynthesis is not a bacterial nitrogen process.


  38. Edward Jenner, Alexander Fleming, Louis Pasteur, Robert Frost
     Answer: Robert Frost
     Explanation: Robert Frost was a poet, others are microbiologists/scientists.


  39. Lactobacillus, Clostridium, Rhizobium, Rust
     Answer: Rust
     Explanation: Rust is a fungal disease, others are bacteria.


  40. Tetanus, Tuberculosis, Cholera, Ringworm
     Answer: Ringworm
     Explanation: Ringworm is fungal, others are bacterial diseases.


  41. Streptomycin, Vaccine, Serum, Antitoxin
     Answer: Streptomycin
     Explanation: Streptomycin is an antibiotic, others give immunity.


  42. Freezing, Salting, Pickling, Putrefaction
     Answer: Putrefaction
     Explanation: Putrefaction spoils food, others preserve food.


  43. Nitrosomonas, Nitrobacter, Rhizobium, Amoeba
     Answer: Amoeba
     Explanation: Amoeba is a protozoan, others are bacteria.


  44. Cheese, Bread, Wine, Rust
     Answer: Rust
     Explanation: Rust is a fungal disease, others are useful microbial products.


  45. Amanita, Agaricus, Penicillium, Yeast
     Answer: Penicillium
     Explanation: Penicillium is mould, others are edible fungi (or yeast).


  46. Anthrax, Cholera, Tuberculosis, AIDS
     Answer: AIDS
     Explanation: AIDS is viral, others are bacterial diseases.


  47. Carbon dioxide, Alcohol, Oxygen, Antibiotic
     Answer: Antibiotic
     Explanation: Antibiotic is not a fermentation product of yeast.


  48. Clostridium, Salmonella, Lactobacillus, Aspergillus
     Answer: Aspergillus
     Explanation: Aspergillus is a fungus, others are bacteria.


  49. Louis Pasteur, Alexander Fleming, Edward Jenner, Isaac Newton
     Answer: Isaac Newton
     Explanation: Newton was a physicist, others worked in microbiology.


  50. Bread, Wine, Beer, Tuberculosis
     Answer: Tuberculosis
     Explanation: Tuberculosis is a disease, others are useful microbial products.

Match the Pair

Set 1

Column A

  1. Rhizobium
  2. Nitrosomonas
  3. Nitrobacter
  4. Pseudomonas
  5. Denitrifying bacteria

Column B
 a. Converts ammonia into nitrites
 b. Reduces soil fertility
 c. Converts nitrites into nitrates
 d. Nitrogen fixation in legumes
 e. Used in sewage treatment

Correct Answers:
 1 – d
 2 – a
 3 – c
 4 – e
 5 – b



Set 2

Column A

  1. Lactobacillus
  2. Clostridium
  3. Acetobacter
  4. Xanthomonas citri
  5. Bacillus anthracis

Column B
 a. Causes citrus canker
 b. Converts alcohol into vinegar
 c. Leather tanning
 d. Causes anthrax
 e. Converts milk sugar into lactic acid

Correct Answers:
 1 – e
 2 – c
 3 – b
 4 – a
 5 – d



Set 3

Column A

  1. Mycobacterium tuberculosis
  2. Salmonella typhi
  3. Vibrio cholerae
  4. Clostridium tetani
  5. Edward Jenner

Column B
 a. Causes typhoid
 b. Causes tuberculosis
 c. Causes cholera
 d. Developed smallpox vaccine
 e. Causes tetanus

Correct Answers:
 1 – b
 2 – a
 3 – c
 4 – e
 5 – d




Set 4

Column A

  1. Louis Pasteur
  2. Alexander Fleming
  3. Selman Waksman
  4. Pasteurisation
  5. Penicillium notatum

Column B
 a. Discovery of penicillin
 b. Process of heating and cooling milk
 c. Discovered streptomycin
 d. Produced antibiotic penicillin
 e. Father of Microbiology

Correct Answers:
 1 – e
 2 – a
 3 – c
 4 – b
 5 – d



Set 5

Column A

  1. Vaccine
  2. Serum
  3. Antitoxin
  4. Toxoid
  5. BCG

Column B
 a. Contains weakened or dead microbes
 b. Used to prevent tuberculosis
 c. Contains ready-made antibodies
 d. Neutralises bacterial toxins
 e. Modified toxin used for vaccination

Correct Answers:
 1 – a
 2 – c
 3 – d
 4 – e
 5 – b



Set 6

Column A

  1. Sun-drying
  2. Freezing
  3. Smoking
  4. Salting
  5. Dehydration

Column B
 a. Low temperature slows bacterial growth
 b. Removes moisture
 c. Preserves meat and fish
 d. Uses sunlight to prevent spoilage
 e. Preserves food by removing water completely

Correct Answers:
 1 – d
 2 – a
 3 – c
 4 – b
 5 – e



Set 7

Column A

  1. Yeast
  2. Saccharomyces cerevisiae
  3. Carbon dioxide
  4. Alcohol fermentation
  5. Budding

Column B
 a. Common baker’s yeast
 b. Gas that makes dough rise
 c. Reproduction in yeast
 d. Converts sugar into alcohol
 e. Used in baking and brewing

Correct Answers:
 1 – e
 2 – a
 3 – b
 4 – d
 5 – c




Set 8

Column A

  1. Wine
  2. Beer
  3. Cheese
  4. Penicillium
  5. Mushroom

Column B
 a. Made by fermenting grapes
 b. Ripening involves fungi
 c. Edible fungus
 d. Fermented cereal grains
 e. Fungus used in antibiotics

Correct Answers:
 1 – a
 2 – d
 3 – b
 4 – e
 5 – c



Set 9

Column A

  1. Agaricus
  2. Amanita
  3. Ringworm
  4. Aspergillus niger
  5. Rhizopus

Column B
 a. Poisonous mushroom
 b. Causes skin disease in humans
 c. Used for citric acid production
 d. Common edible mushroom
 e. Black bread mould

Correct Answers:
 1 – d
 2 – a
 3 – b
 4 – c
 5 – e


Set 10

Column A

  1. Nitrogen cycle
  2. Nitrification
  3. Denitrification
  4. Nitrogen fixation
  5. Putrefaction

Column B
 a. Conversion of ammonia to nitrites/nitrates
 b. Conversion of nitrates into nitrogen gas
 c. Process of nitrogen circulation in nature
 d. Spoilage of proteins by bacteria
 e. Conversion of atmospheric nitrogen into compounds

Correct Answers:
 1 – c
 2 – a
 3 – b
 4 – e
 5 – d

Short Answer Questions  

  1. Which antibiotic was discovered by Alexander Fleming?
     Answer: Alexander Fleming discovered the antibiotic penicillin from the fungus Penicillium notatum.


  2. What are vaccines?
     Answer: Vaccines are preparations of weakened or killed germs that stimulate the body to develop immunity against a disease.


  3. What is the role of serums in medicine?
     Answer: Serums provide ready-made antibodies that give immediate passive immunity to the patient.


  4. What are toxoids?
     Answer: Toxoids are chemically treated bacterial toxins that lose their harmful effect but still stimulate immunity.


  5. What is the role of nitrogen-fixing bacteria?
     Answer: Nitrogen-fixing bacteria convert free nitrogen of the atmosphere into nitrogen compounds usable by plants.


  6. Give one example of a symbiotic nitrogen-fixing bacterium.
     Answer: Rhizobium lives in root nodules of legumes and fixes nitrogen for the plant.


  7. What is the function of nitrifying bacteria?
     Answer: Nitrifying bacteria convert ammonia into nitrites and nitrates which can be absorbed by plants.


  8. What is the role of denitrifying bacteria?
     Answer: Denitrifying bacteria break down nitrates into free nitrogen, reducing soil fertility.


  9. How are bacteria used in leather tanning?
     Answer: Certain bacteria decompose organic matter and help in softening and tanning of hides to make leather.


  10. How do bacteria help in tea production?
     Answer: Bacteria help in fermentation during curing of tea leaves which improves flavour and aroma.


  11. Name a bacterium used in vinegar production and its role.
     Answer: Acetobacter aceti converts alcohol into acetic acid which is used in vinegar production.


  12. How does Lactobacillus help in milk products?
     Answer: Lactobacillus converts lactose in milk into lactic acid leading to curdling of milk.


  13. What is anthrax and which organism causes it?
     Answer: Anthrax is a fatal disease of cattle caused by the bacterium Bacillus anthracis.


  14. What is citrus canker?
     Answer: Citrus canker is a bacterial disease of citrus plants caused by Xanthomonas citri.


  15. What is ring rot of potato?
     Answer: Ring rot of potato is a bacterial disease caused by Corynebacterium sepedonicum.


  16. How is tuberculosis transmitted to humans from animals?
     Answer: Tuberculosis can spread to humans through infected milk from diseased cattle.


  17. What is brucellosis?
     Answer: Brucellosis is a bacterial disease of sheep and goats that causes abortion and fever.


  18. How does pasteurisation prevent bacterial spoilage of milk?
     Answer: Pasteurisation kills harmful bacteria in milk by heating it without affecting taste or nutrition.


  19. How does yeast help in bread making?
     Answer: Yeast ferments sugar to produce carbon dioxide which makes bread soft and spongy.


  20. What makes bread rise during baking?
     Answer: The carbon dioxide released by yeast during fermentation causes bread to rise.


  21. Which fungus produces penicillin and how is it used?
     Answer: Penicillium notatum produces penicillin which is used as an antibiotic to kill harmful bacteria.


  22. What is streptomycin and its source?
     Answer: Streptomycin is an antibiotic produced by the bacterium Streptomyces griseus.


  23. How does Penicillium help in cheese production?
     Answer: Penicillium species grow in cheese and give it special flavour and texture.


  24. How is wine produced?
     Answer: Wine is produced by fermenting sugars in grapes with yeast to form alcohol.


  25. Which edible fungus is cultivated on a large scale and why?
     Answer: Mushrooms like Agaricus are cultivated as they are highly nutritious and rich in protein.


  26. What is the nutritive value of mushrooms?
     Answer: Mushrooms are rich in proteins, vitamins, and minerals but low in fats.


  27. How is soy sauce prepared?
     Answer: Soy sauce is prepared by fermenting soybeans with the fungus Aspergillus oryzae.


  28. Which fungus causes spoilage of bread?
     Answer: Bread is spoiled by Rhizopus which grows as black mould.


  29. What is ringworm and what causes it?
     Answer: Ringworm is a fungal disease of human skin caused by Trichophyton.


  30. How do bacteria help in buttermilk preparation?
     Answer: Lactic acid bacteria ferment milk sugars and convert milk into buttermilk.


  31. What is BCG vaccine used for?
     Answer: The BCG vaccine is used to prevent tuberculosis.


  32. What is DPT vaccine used for?
     Answer: The DPT vaccine protects against diphtheria, pertussis, and tetanus.


  33. What are antitoxins?
     Answer: Antitoxins are ready-made antibodies produced against toxins to provide immediate passive immunity.


  34. How is tetanus toxoid prepared?
     Answer: Tetanus toxoid is prepared by chemically treating tetanus toxin to make it harmless.


  35. What is the role of Aspergillus niger in industry?
     Answer: Aspergillus niger is used in the large-scale production of citric acid.


  36. Why is Aspergillus flavus harmful?
     Answer: Aspergillus flavus produces aflatoxins which are poisonous to humans and animals.


  37. How does Clostridium botulinum cause food poisoning?
     Answer: Clostridium botulinum grows in improperly canned food and produces a deadly toxin.


  38. What is lockjaw and what causes it?
     Answer: Lockjaw is another name for tetanus caused by Clostridium tetani.


  39. How do fungi affect wheat crops?
     Answer: Fungi cause rust disease in wheat which reduces crop yield.


  40. What is the common name of Agaricus bisporus?
     Answer: The common name of Agaricus bisporus is button mushroom.


  41. How is alcohol made from molasses?
     Answer: Yeast ferments sugars present in molasses to produce alcohol.


  42. How does pickling prevent bacterial spoilage?
     Answer: Pickling uses salt or vinegar which creates conditions unsuitable for bacterial growth.


  43. How does Lactobacillus convert milk to curd?
     Answer: Lactobacillus ferments lactose in milk to form lactic acid, which coagulates proteins and sets curd.


  44. How is Roquefort cheese ripened?
     Answer: Roquefort cheese is ripened by the fungus Penicillium roqueforti.


  45. How is Swiss cheese produced?
     Answer: Swiss cheese is produced using Propionibacterium shermanii which releases CO₂ and forms characteristic holes.


  46. Why is yeast used in the baking industry?
     Answer: Yeast produces carbon dioxide during fermentation which makes dough rise in baking.


  47. Which fungus is cultivated as food?
     Answer: Edible mushrooms like Agaricus campestris are cultivated for food.


  48. What is citrus canker and its cause?
     Answer: Citrus canker is a bacterial disease of citrus plants caused by Xanthomonas citri.


  49. Which antibiotic is obtained from Cephalosporium?
     Answer: The fungus Cephalosporium produces the antibiotic cephalosporin.


  50. What is the importance of yeast in brewing?
     Answer: Yeast ferments sugars in fruit juices or grains to produce alcohol in brewing industries.

Puzzles

Q.1. I live in the root nodules of legumes and fix nitrogen. Who am I?
 Ans. Rhizobium

Q.2. I convert ammonia into nitrites. Name me.
 Ans. Nitrosomonas

Q.3. I convert nitrites into nitrates. Who am I?
 Ans. Nitrobacter

Q.4. I reduce soil nitrates back into nitrogen gas. Who am I?
 Ans. Denitrifying bacteria (Pseudomonas)

Q.5. I am the first antibiotic discovered, produced by a fungus. Who am I?
 Ans. Penicillin

Q.6. I am a weakened toxin used in vaccines. Who am I?
 Ans. Toxoid

Q.7. I provide immediate immunity as I contain ready-made antibodies. Who am I?
 Ans. Serum

Q.8. I neutralize bacterial toxins inside the body. Who am I?
 Ans. Antitoxin

Q.9. I turn milk into curd by producing lactic acid. Who am I?
 Ans. Lactobacillus

Q.10. I am used to make bread spongy. Who am I?
 Ans. Yeast (Saccharomyces cerevisiae)

Q.11. I give flavour to tea leaves after fermentation. Who am I?
 Ans. Bacteria and fungi during tea fermentation

Q.12. I soften animal hides in leather tanning. Who am I?
 Ans. Bacteria

Q.13. I cause citrus canker in oranges and lemons. Who am I?
 Ans. Xanthomonas citri

Q.14. I cause ring disease in potatoes. Who am I?
 Ans. Clavibacter

Q.15. I am a fungal food rich in protein, often called vegetarian’s meat. Who am I?
 Ans. Mushroom (Agaricus)

Q.16. I am a fermented drink made from grapes. Who am I?
 Ans. Wine

Q.17. I am the bacterium that causes anthrax in cattle. Who am I?
 Ans. Bacillus anthracis

Q.18. I am a bacterial disease that reduces milk yield in cattle. Who am I?
 Ans. Tuberculosis (Mycobacterium bovis)

Q.19. I am a fungus that produces aflatoxins in stored grains. Who am I?
 Ans. Aspergillus flavus

Q.20. I am a fungal antibiotic other than penicillin. Who am I?
 Ans. Cephalosporin

Q.21. I am added to dough to make it rise. Who am I?
 Ans. Yeast

Q.22. I am the chemical process yeast uses to make alcohol. What am I?
 Ans. Fermentation

Q.23. I am the gas produced by yeast in bread-making. Who am I?
 Ans. Carbon dioxide

Q.24. I am used to preserve jams by reducing water content. Who am I?
 Ans. Sugar

Q.25. I am used in pickles to prevent bacterial growth. Who am I?
 Ans. Salt

Q.26. I am the process of heating milk to kill bacteria. Who am I?
 Ans. Pasteurization

Q.27. I am a bacterial vaccine used to protect against TB. Who am I?
 Ans. BCG vaccine

Q.28. I am a toxoid vaccine used against diphtheria. Who am I?
 Ans. DPT vaccine

Q.29. I am the fungus that ripens Roquefort cheese. Who am I?
 Ans. Penicillium roqueforti

Q.30. I am the product formed when milk proteins coagulate. Who am I?
 Ans. Curd

Q.31. I am responsible for souring of milk. Who am I?
 Ans. Lactic acid bacteria

Q.32. I am used as a preservative in vinegar. Who am I?
 Ans. Acetic acid (produced by Acetobacter)

Q.33. I am a fungal infection of bread. Who am I?
 Ans. Bread mould (Rhizopus)

Q.34. I am a fungus used in brewing industry. Who am I?
 Ans. Yeast

Q.35. I am a fungus used in the production of penicillin. Who am I?
 Ans. Penicillium notatum

Q.36. I am a food rich in proteins, vitamins, and minerals but low in calories. Who am I?
 Ans. Mushroom

Q.37. I am the organism responsible for foul smell in meat and fish spoilage. Who am I?
 Ans. Bacteria

Q.38. I am the bacterial process that adds nitrogen to soil. Who am I?
 Ans. Nitrogen fixation

Q.39. I am the bacterial process that removes nitrogen from soil. Who am I?
 Ans. Denitrification

Q.40. I am the bacterium that lives freely in soil and fixes nitrogen. Who am I?
 Ans. Azotobacter

Q.41. I am the bacterium used in preparation of vinegar. Who am I?
 Ans. Acetobacter aceti

Q.42. I am the fungal structure used for mushroom cultivation. Who am I?
 Ans. Mycelium

Q.43. I am a fungus eaten as food and also used in soups. Who am I?
 Ans. Mushroom

Q.44. I am the bacterium that helps fermenting coffee and cocoa. Who am I?
 Ans. Lactic acid bacteria

Q.45. I am the method of preserving food by freezing. Who am I?
 Ans. Refrigeration

Q.46. I am the immediate immune response given by injecting antibodies. Who am I?
 Ans. Passive immunity (serum therapy)

Q.47. I am a harmful bacterium that can spoil canned food. Who am I?
 Ans. Clostridium botulinum

Q.48. I am the useful role of yeast in baking, brewing, and wine-making. Who am I?
 Ans. Fermentation

Q.49. I am the nutritive value of mushrooms that makes them valuable. What am I?
 Ans. Rich source of proteins, vitamins, and minerals

Q.50. I am the group of organisms including bacteria and fungi that have great economic importance. Who am I?
 Ans. Microorganisms

Difference Between:

Q.1. Difference between useful and harmful bacteria
 Ans.

  • Useful bacteria: Rhizobium, Lactobacillus – used in nitrogen fixation, curd preparation, antibiotics, and food industry.
  • Harmful bacteria: Xanthomonas, Clostridium – cause plant and human diseases and spoil food.

 

Q.2. Difference between antibiotics and antitoxins
 Ans.

  • Antibiotics: Substances produced by microorganisms (fungi/bacteria) that kill or inhibit bacteria; used to treat infections (e.g., Penicillin).
  • Antitoxins: Antibodies produced against bacterial toxins; neutralize toxins but do not kill bacteria directly.

 

Q.3. Difference between vaccine and toxoid
 Ans.

  • Vaccine: Contains weakened or killed microorganisms to stimulate immunity.
  • Toxoid: Inactivated bacterial toxin that produces immunity without causing disease (e.g., tetanus toxoid).

 

Q.4. Difference between nitrogen-fixing and nitrifying bacteria
 Ans.

  • Nitrogen-fixing bacteria: Convert atmospheric nitrogen into ammonia/nitrates usable by plants (Rhizobium, Azotobacter).
  • Nitrifying bacteria: Convert ammonia into nitrites and then nitrates (Nitrosomonas, Nitrobacter).

 

Q.5. Difference between nitrifying and denitrifying bacteria
 Ans.

  • Nitrifying bacteria: Increase soil fertility by producing nitrates from ammonia.
  • Denitrifying bacteria: Reduce soil fertility by converting nitrates back into atmospheric nitrogen (Pseudomonas).

 

Q.6. Difference between yeast and bacteria
 Ans.

  • Yeast: Eukaryotic fungus, used in baking, brewing, and wine-making; facultative anaerobe.
  • Bacteria: Prokaryotic organism, used in medicine, agriculture, and industry; can be aerobic or anaerobic.

 

Q.7. Difference between curd and cheese
 Ans.

  • Curd: Formed by bacterial fermentation of milk (Lactobacillus), semi-solid, mildly sour.
  • Cheese: Prepared by bacterial or fungal fermentation of milk; solid, aged, has characteristic flavour (Penicillium).

 

Q.8. Difference between useful fungi and harmful fungi
 Ans.

  • Useful fungi: Produce antibiotics, alcohol, bread, cheese; used in mushroom cultivation.
  • Harmful fungi: Cause food spoilage, plant diseases, and produce toxins (e.g., Aspergillus).

 

Q.9. Difference between alcoholic fermentation and lactic acid fermentation
 Ans.

  • Alcoholic fermentation: Yeast converts sugars into ethanol + CO₂; used in wine and beer.
  • Lactic acid fermentation: Lactobacillus converts sugars into lactic acid; used in curd and yoghurt.

 

Q.10. Difference between serums and vaccines
 Ans.

  • Serums: Contain ready-made antibodies; provide immediate passive immunity.
  • Vaccines: Contain weakened/killed microorganisms; induce active immunity over time.

 

Q.11. Difference between spoilage of food by bacteria and fungi
 Ans.

  • Bacteria: Produce foul odour, gas, and slime (e.g., milk spoilage).
  • Fungi: Form moulds or mycelium on food surfaces (e.g., bread mould, grains).

 

Q.12. Difference between wine and beer production
 Ans.

  • Wine: Fermented from fruit juice (grapes) by yeast, primarily alcoholic content.
  • Beer: Fermented from cereals (barley/wheat) by yeast, contains alcohol + foam due to CO₂.

 

Q.13. Difference between edible mushrooms and poisonous fungi
 Ans.

  • Edible mushrooms: Non-toxic, nutritious, used as food (Agaricus).
  • Poisonous fungi: Toxic, can cause illness or death (Amanita species).

 

Q.14. Difference between active immunity and passive immunity
 Ans.

  • Active immunity: Produced when body generates antibodies after vaccination or infection; long-lasting.
  • Passive immunity: Received from external source (serum/antitoxin); immediate but short-lived.

 

Q.15. Difference between pasteurization and refrigeration
 Ans.

  • Pasteurization: Heating milk to kill harmful bacteria; kills only pathogens, not all microbes.
  • Refrigeration: Lowers temperature to slow bacterial growth; does not kill bacteria.

 

Q.16. Difference between soil bacteria and pathogenic bacteria
 Ans.

  • Soil bacteria: Useful, enrich soil fertility (Rhizobium, Azotobacter).
  • Pathogenic bacteria: Harmful, cause diseases in plants and animals (Clavibacter, Bacillus anthracis).

 

Q.17. Difference between active and dormant bacterial forms
 Ans.

  • Active form: Bacteria are metabolically active, multiply rapidly.
  • Dormant form: Bacteria form spores under harsh conditions, remain inactive until conditions improve.

 

Q.18. Difference between sericulture and mushroom cultivation
 Ans.

  • Sericulture: Rearing silkworms for silk production; insects involved.
  • Mushroom cultivation: Growing edible fungi for food; fungus involved.

 

Q.19. Difference between bacterial and fungal antibiotics
 Ans.

  • Bacterial antibiotics: Produced by bacteria (e.g., Streptomycin by Streptomyces).
  • Fungal antibiotics: Produced by fungi (e.g., Penicillin by Penicillium).

 

Q.20. Difference between active and passive role of microbes in industry
 Ans.

  • Active role: Microbes participate directly in fermentation (yeast in bread, wine).
  • Passive role: Microbial enzymes used in leather tanning or flavour development without growth.

Assertion and Reason

  1.  

Assertion: Rhizobium is useful to farmers.
 Reason: It causes root nodules in leguminous plants and fixes atmospheric nitrogen.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Nitrosomonas helps in nitrification.
 Reason: It converts ammonia into nitrites.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Nitrobacter is harmful to agriculture.
 Reason: It converts nitrites into nitrates.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Lactobacillus is used in making curd.
 Reason: It converts lactose sugar of milk into lactic acid.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Yeast is used in bakeries.
 Reason: Yeast produces carbon dioxide which makes the dough rise.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Yeast respires aerobically during alcohol production.
 Reason: In absence of oxygen, yeast produces alcohol and carbon dioxide.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Aspergillus niger is used in citric acid production.
 Reason: It is a pathogenic bacterium.
 Answer: Assertion is true, Reason is false.

  1.  

Assertion: Vinegar is produced by Acetobacter.
 Reason: Acetobacter converts alcohol into acetic acid.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Penicillin is an antibiotic obtained from Penicillium notatum.
 Reason: It kills or inhibits the growth of harmful bacteria.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Antibiotics are used to treat viral diseases.
 Reason: Antibiotics kill bacteria but not viruses.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Edward Jenner is called the “Father of Immunology”.
 Reason: He discovered the smallpox vaccine.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Louis Pasteur disproved spontaneous generation.
 Reason: He proved that microorganisms come only from pre-existing microorganisms.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Pasteurisation prevents milk spoilage.
 Reason: In pasteurisation, milk is heated to 62°C for 30 minutes or 72°C for 15 seconds and then cooled rapidly.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Smoking and salting preserve fish and meat.
 Reason: They make the environment unsuitable for bacterial growth.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Freezing preserves food.
 Reason: Low temperature stops bacterial growth completely.
 Answer: Assertion is true, Reason is false.

  1.  

Assertion: Sun-drying prevents food spoilage.
 Reason: It removes moisture which bacteria and fungi need to grow.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Cholera is a viral disease.
 Reason: It is caused by Vibrio cholerae, a bacterium.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Tuberculosis is caused by Mycobacterium tuberculosis.
 Reason: It affects the lungs in humans.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Typhoid is spread by contaminated water and food.
 Reason: It is caused by Salmonella typhi.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Citrus canker is a fungal disease.
 Reason: It is caused by Xanthomonas citri, a bacterium.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Tetanus is caused by Clostridium tetani.
 Reason: The bacterium enters through wounds.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Anthrax affects humans and cattle.
 Reason: It is caused by Bacillus anthracis.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Ringworm is caused by bacteria.
 Reason: It is actually caused by a fungus.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Rust of wheat is caused by a virus.
 Reason: It is a fungal disease.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Polio can be prevented by vaccination.
 Reason: Vaccines stimulate the body to produce immunity.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Vaccines provide active immunity.
 Reason: They stimulate the body to produce antibodies.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Serum provides active immunity.
 Reason: Serum contains ready-made antibodies.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Antitoxins neutralise toxins produced by bacteria.
 Reason: They are antibodies produced against bacterial toxins.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Toxoids are inactivated toxins.
 Reason: They are used for vaccination.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Putrefaction is useful for food preservation.
 Reason: It spoils food by bacterial action.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Fermentation is used in alcohol production.
 Reason: Yeast converts sugars into alcohol and carbon dioxide.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Yeast is used in making antibiotics.
 Reason: Yeast produces alcohol, not antibiotics.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Cheese making involves bacteria.
 Reason: Lactic acid bacteria convert lactose into lactic acid and help curd formation.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Yeast is used in beer and wine making.
 Reason: Yeast ferments sugar in grains and fruits.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Rust, Smut and Ringworm are bacterial diseases.
 Reason: They are fungal diseases.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Antibiotics are produced only by fungi.
 Reason: Some antibiotics are produced by bacteria too.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Fleming discovered Penicillin.
 Reason: He observed Penicillium fungus inhibiting bacterial growth.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Jenner developed vaccination for rabies.
 Reason: Pasteur developed rabies vaccine, not Jenner.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Nitrogen fixation improves soil fertility.
 Reason: Nitrogen-fixing bacteria convert atmospheric nitrogen into usable compounds.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Denitrifying bacteria are useful for agriculture.
 Reason: They reduce soil fertility by converting nitrates to nitrogen gas.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Antibiotics are chemical substances secreted by microorganisms.
 Reason: They can destroy or inhibit the growth of other microorganisms.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Alcoholic fermentation is an aerobic process.
 Reason: It occurs in absence of oxygen.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Lactic acid bacteria spoil milk.
 Reason: They curdle milk and are useful in curd preparation.
 Answer: Assertion is true, Reason is true, but Reason does not explain Assertion.

  1.  

Assertion: Smoking kills bacteria in food.
 Reason: Smoke creates a dry, antimicrobial environment around the food.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Freezing kills bacteria permanently.
 Reason: Freezing only slows down their growth but does not kill them.
 Answer: Assertion is false, Reason is true.

  1.  

Assertion: Diseases like Tuberculosis and Typhoid are communicable.
 Reason: They spread from one person to another.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Vaccination provides quick relief in infections.
 Reason: Vaccines act immediately.
 Answer: Both Assertion and Reason are false.

  1.  

Assertion: Antibiotics can be misused.
 Reason: Overuse makes bacteria resistant to them.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: Some fungi are edible.
 Reason: Mushrooms like Agaricus are eaten as food.
 Answer: Both Assertion and Reason are true, and Reason correctly explains Assertion.

  1.  

Assertion: All bacteria are harmful to humans.
 Reason: Some bacteria are useful in food, medicine, and agriculture.
 Answer: Assertion is false, Reason is true.

True or False

  1. Rhizobium helps in nitrogen fixation in leguminous plants.
     Answer: True


  2. Nitrifying bacteria reduce soil fertility.
     Answer: False (They improve fertility by converting ammonia into nitrites/nitrates)


  3. Denitrifying bacteria are useful for agriculture.
     Answer: False (They reduce soil fertility)


  4. Nitrosomonas converts ammonia into nitrites.
     Answer: True


  5. Lactobacillus converts milk sugar into lactic acid.
     Answer: True


  6. Clostridium is used in leather tanning.
     Answer: True


  7. Acetobacter converts alcohol into vinegar.
     Answer: True


  8. Citrus canker is a fungal disease.
     Answer: False (It is caused by a bacterium)


  9. Tuberculosis is caused by Mycobacterium tuberculosis.
     Answer: True


  10. Typhoid is caused by a virus.
     Answer: False (It is caused by a bacterium)


  11. Tetanus is caused by Clostridium tetani.
     Answer: True


  12. Anthrax is a disease caused by Bacillus anthracis.
     Answer: True


  13. Alexander Fleming discovered streptomycin.
     Answer: False (He discovered penicillin)


  14. Selman Waksman discovered streptomycin.
     Answer: True


  15. Edward Jenner discovered the vaccine for rabies.
     Answer: False (He developed smallpox vaccine)


  16. Louis Pasteur developed the rabies vaccine.
     Answer: True


  17. Vaccines provide passive immunity.
     Answer: False (They provide active immunity)


  18. Serums provide passive immunity.
     Answer: True


  19. Antitoxins are antibodies that neutralise toxins.
     Answer: True


  20. Toxoids are modified toxins used as vaccines.
     Answer: True


  21. Pasteurisation involves heating milk at high temperature and cooling rapidly.
     Answer: True


  22. Putrefaction is useful for food preservation.
     Answer: False (It spoils food)


  23. Freezing kills bacteria permanently.
     Answer: False (It slows growth but does not kill them)


  24. Drying removes moisture and prevents bacterial growth.
     Answer: True


  25. Salting helps preserve fish and meat.
     Answer: True


  26. Smoking is used to preserve meat.
     Answer: True


  27. Yeast is a multicellular fungus.
     Answer: False (It is unicellular)


  28. Yeast reproduces by budding.
     Answer: True


  29. Saccharomyces cerevisiae is called brewer’s yeast.
     Answer: True


  30. Yeast respires anaerobically during alcohol fermentation.
     Answer: True


  31. Carbon dioxide makes bread spongy and soft.
     Answer: True


  32. Wine is produced by fermenting grapes with yeast.
     Answer: True


  33. Beer is produced from fruits like grapes.
     Answer: False (Beer is made from cereals)


  34. Cheese ripening involves bacteria and fungi.
     Answer: True


  35. Penicillium is used in the ripening of some cheeses.
     Answer: True


  36. Penicillin is an antibiotic obtained from bacteria.
     Answer: False (It is obtained from fungus)


  37. Streptomycin is obtained from Streptomyces.
     Answer: True


  38. Agaricus is an edible mushroom.
     Answer: True


  39. Amanita is a common edible mushroom.
     Answer: False (It is poisonous)


  40. Ringworm is caused by bacteria.
     Answer: False (It is caused by fungi)


  41. Rust of wheat is a fungal disease.
     Answer: True


  42. Aspergillus niger is used to produce citric acid.
     Answer: True


  43. Rhizopus is commonly known as black bread mould.
     Answer: True


  44. Yeast is used in the production of antibiotics.
     Answer: False (It is used in baking and alcohol production)


  45. Nitrogen cycle maintains soil fertility.
     Answer: True


  46. Denitrification converts nitrates into nitrogen gas.
     Answer: True


  47. Vaccines provide immediate relief from infections.
     Answer: False (They take time to develop immunity)


  48. Overuse of antibiotics can make bacteria resistant.
     Answer: True


  49. Mushrooms are rich in proteins and vitamins.
     Answer: True


  50. All fungi are harmful to humans.
     Answer: False (Many fungi are useful)

Long Answer Questions

  1. Explain the role of bacteria in medicine.
     Answer: Bacteria play an important role in medicine as they are used in the preparation of antibiotics, vaccines, sera, antitoxins, and toxoids. Antibiotics like streptomycin are obtained from bacteria such as Streptomyces. Vaccines made from killed or weakened bacteria help the body build immunity. Sera contain ready-made antibodies for immediate protection. Antitoxins neutralise bacterial toxins, and toxoids are chemically modified toxins that provide long-term immunity.


  2. What are antibiotics? Give examples.
     Answer: Antibiotics are chemical substances produced by certain microbes which can kill or inhibit the growth of harmful bacteria without damaging human tissues. They are widely used to treat bacterial infections. Examples include penicillin from the fungus Penicillium notatum, streptomycin from Streptomyces griseus, and tetracycline from Streptomyces aureofaciens.


  3. Explain what vaccines are and how they work.
     Answer: Vaccines are preparations of killed or weakened germs which, when introduced into the body, stimulate the immune system to produce antibodies against specific diseases. They provide active immunity, meaning the body learns to fight the disease itself. For example, BCG vaccine is used against tuberculosis and DPT vaccine protects against diphtheria, pertussis, and tetanus.


  4. Differentiate between serum and vaccine.
     Answer: A serum is a preparation containing ready-made antibodies that provides immediate passive immunity, useful in emergencies like snake bite or tetanus exposure. A vaccine, on the other hand, contains weakened or killed germs which stimulate the body to develop its own antibodies, thus providing long-lasting active immunity.


  5. What are toxoids? Give examples.
     Answer: Toxoids are bacterial toxins that have been chemically treated so that they lose their harmful effect but still retain the ability to stimulate antibody formation. They are used in vaccines to protect against diseases caused by bacterial toxins. Examples include diphtheria toxoid and tetanus toxoid.


  6. Explain the role of nitrogen-fixing bacteria in agriculture.
     Answer: Nitrogen-fixing bacteria convert free nitrogen gas from the atmosphere into nitrogen compounds like nitrates and ammonia which plants can use. Some like Rhizobium live symbiotically in root nodules of leguminous plants, while free-living forms like Azotobacter and Clostridium fix nitrogen directly in the soil. This natural process enriches soil fertility and reduces the need for artificial fertilizers.


  7. Describe nitrifying bacteria and their importance.
     Answer: Nitrifying bacteria are soil bacteria that convert ammonia into nitrites and then nitrates, which are easily absorbed by plants. Nitrosomonas converts ammonia into nitrites, while Nitrobacter converts nitrites into nitrates. This process, known as nitrification, is an essential part of the nitrogen cycle that maintains soil fertility.


  8. What is the role of denitrifying bacteria in nature?
     Answer: Denitrifying bacteria like Pseudomonas convert nitrates in the soil back into free atmospheric nitrogen. While this reduces soil fertility by removing nitrates, it plays an important role in maintaining the balance of nitrogen in the atmosphere.


  9. How do bacteria help in leather tanning?
     Answer: In the leather industry, bacteria are used to decompose unwanted proteins, hair, and flesh from animal hides. Bacterial action softens and cleans the hides, making them suitable for tanning. The tanned leather then becomes durable, flexible, and useful for making goods like shoes, belts, and bags.


  10. Explain the role of bacteria in the tea industry.
     Answer: Certain bacteria help in the fermentation process of curing tea leaves, which enhances flavour and aroma. Bacterial enzymes modify the chemical composition of tea leaves, reducing bitterness and improving the overall quality of the beverage.


  11. How do bacteria cause food spoilage?
     Answer: Bacteria decompose proteins, fats, and carbohydrates present in food, producing unpleasant odour, taste, and toxins. For example, Clostridium botulinum in canned food produces dangerous toxins causing botulism. Spoiled food can lead to serious illnesses if consumed.


  12. Describe three methods of food preservation against bacteria.
     Answer:


  • Refrigeration: Slows down bacterial growth by keeping food at low temperature.
  • Pasteurisation: Destroys harmful bacteria by heating milk and cooling it rapidly.
  • Pickling/Salting: Uses vinegar or salt to create an environment where bacteria cannot grow.
  1. Name some bacterial diseases in animals and their effects.
    Answer: Cattle suffer from anthrax caused by Bacillus anthracis, which is often fatal. Sheep and goats suffer from brucellosis causing abortion and weakness. Tuberculosis affects cattle and may be transmitted to humans through infected milk. These diseases reduce livestock health and productivity.


  2. What are bacterial diseases in plants? Give examples.
    Answer: Bacterial diseases in plants damage crops, leading to poor yield and economic loss. Citrus canker in citrus fruits is caused by Xanthomonas citri. Potato suffers from ring rot caused by Corynebacterium sepedonicum. Such diseases destroy food crops and cause great agricultural losses.


  3. Describe the economic importance of fungi in medicine.
    Answer: Fungi produce important antibiotics like penicillin from Penicillium notatum, which kills harmful bacteria. Streptomycin from Streptomyces also fights bacterial infections. These fungal antibiotics have revolutionised medicine by curing deadly diseases like pneumonia, tuberculosis, and syphilis.


  4. Explain the role of yeast in the baking industry.
    Answer: Yeast ferments sugar in dough, releasing carbon dioxide gas which gets trapped in the dough, making it rise. During baking, the gas escapes, leaving the bread soft, spongy, and porous. Thus, yeast is essential in bread and cake making.


  5. How is wine produced using fungi?
    Answer: Wine is produced by fermenting sugars present in grape juice with yeast (Saccharomyces cerevisiae). Yeast enzymes convert sugars into alcohol and carbon dioxide. The alcohol remains in the liquid, making it an alcoholic beverage.


  6. Describe the role of fungi in cheese production.
    Answer: In cheese-making, fungi such as Penicillium roqueforti grow inside the cheese and produce enzymes that give it unique flavours and textures. Some cheeses like Roquefort and Camembert owe their distinct taste to fungal fermentation.


  7. What is mushroom cultivation and its importance?
    Answer: Mushrooms are edible fungi like Agaricus bisporus that are cultivated under controlled conditions. They are rich in proteins, vitamins, and minerals, making them highly nutritious. Mushroom cultivation is also an important industry providing food and employment.


  8. What is the nutritive value of mushrooms?
    Answer: Mushrooms are high in protein, contain essential amino acids, vitamins like B-complex and vitamin D, and minerals like potassium and phosphorus. They are low in fat and calories, making them a healthy food option.



  1. Explain the process of leather tanning by bacteria.
    Leather tanning is the process of treating animal hides to make them durable and resistant to decay. Certain bacteria play an important role in tanning by decomposing the organic matter, softening the hides, and removing hair from them. Bacterial enzymes help in the breakdown of proteins and fats present in the skin, making it pliable and suitable for tanning. This biological method is considered eco-friendly compared to chemical methods.



  1. How do bacteria contribute to the flavour of tea?
    During the fermentation of tea leaves, bacteria and fungi act on the chemical constituents of the leaves, particularly polyphenols. The microbial activity brings about biochemical changes that develop the characteristic aroma and flavour of tea. Without the bacterial role in fermentation, tea would lack its distinct taste and quality.



  1. Explain the spoilage of food caused by bacteria.
    Food spoilage occurs when bacteria multiply in food, breaking down proteins, carbohydrates, and fats. This results in bad smell, sour taste, discoloration, and sometimes production of harmful toxins. For example, milk turns sour due to bacterial action, while meat and fish produce foul odours. Spoiled food becomes unsafe for consumption as it may cause food poisoning.



  1. What are the preventive measures to avoid food spoilage by bacteria?
    Preventive measures include refrigeration and freezing to slow down bacterial growth, pasteurization to kill harmful bacteria in milk, canning and bottling to prevent contamination, salting and sugaring to reduce water content in food, and using preservatives such as vinegar. Proper hygiene and airtight storage are also important for reducing bacterial spoilage.



  1. Discuss the harmful effects of bacteria on plants.
    Bacteria cause several plant diseases leading to reduced yield and economic loss. Examples include citrus canker in lemon and orange caused by Xanthomonas citri, and ring disease of potatoes caused by Clavibacter. These diseases damage leaves, fruits, and stems, resulting in poor quality and quantity of produce. Such bacterial infections can spread rapidly, affecting entire crops.



  1. Describe two bacterial diseases of animals.
    (i) Anthrax – caused by Bacillus anthracis, affects cattle, sheep, and goats; symptoms include fever, swelling, and difficulty in breathing.
     (ii) Tuberculosis – caused by Mycobacterium bovis in cattle; leads to weakness, weight loss, and reduced milk production. These diseases spread to humans in some cases, making them zoonotic.



  1. State the economic importance of yeast in alcohol production.
    Yeast, especially Saccharomyces cerevisiae, plays a vital role in alcohol production. It ferments sugars into alcohol and carbon dioxide. This property is used in making beer, wine, whisky, and other alcoholic beverages. The process of fermentation by yeast is cheap and efficient, making it one of the most economically important microorganisms.



  1. How is yeast used in baking industry?
    In baking, yeast ferments the sugar present in dough to release carbon dioxide, which gets trapped in the gluten network of flour, causing the dough to rise and become spongy. This gives bread and cakes their soft texture. Yeast also adds flavour to baked goods through fermentation products.



  1. Explain the role of fungi in the production of antibiotics.
    Certain fungi produce substances called antibiotics that inhibit the growth of harmful bacteria. For example, Penicillium notatum produces penicillin, the first widely used antibiotic. These antibiotics revolutionized medicine by treating previously fatal bacterial infections. Other fungi like Cephalosporium also produce useful antibiotics.



  1. What is the nutritive value of mushrooms?
    Mushrooms are rich in proteins, vitamins (especially B-complex and vitamin D), and minerals such as potassium, iron, and phosphorus. They are low in fat and calories, making them suitable for healthy diets. Mushrooms also contain dietary fibres that aid digestion. Because of this high nutritive value, mushrooms are often considered a vegetarian alternative to meat.



  1. Describe the role of yeast in cheese production.
    In cheese making, microorganisms including fungi and bacteria play a crucial role. Yeast, along with lactic acid bacteria, helps in fermentation and curdling of milk. It aids in breaking down lactose into lactic acid, which helps coagulate milk proteins. Certain types of cheese, like Roquefort and Camembert, also use fungi to develop characteristic flavour, aroma, and texture. Without the action of yeast and fungi, cheese would lack its unique properties.



  1. Explain mushroom cultivation as an industry.
    Mushroom cultivation is an important agro-based industry. It requires controlled conditions of temperature, humidity, and ventilation. Mushrooms are grown on substrates like straw, compost, or wood. They are harvested after fruiting bodies develop. Mushroom farming is profitable as it requires little space, grows quickly, and provides a nutritious food source. It also offers employment opportunities in rural areas.



  1. How do nitrifying bacteria help in agriculture?
    Nitrifying bacteria such as Nitrosomonas and Nitrobacter convert ammonia into nitrites and then into nitrates. These nitrates are absorbed by plants for protein synthesis and growth. This natural process enriches soil fertility and reduces the need for chemical fertilizers. Thus, nitrifying bacteria are crucial for maintaining the nitrogen cycle in agriculture.



  1. Distinguish between nitrogen-fixing bacteria and denitrifying bacteria.
    Nitrogen-fixing bacteria, like Rhizobium and Azotobacter, convert atmospheric nitrogen into ammonia and nitrates, enriching soil fertility and helping plants. On the other hand, denitrifying bacteria such as Pseudomonas convert nitrates back into atmospheric nitrogen, reducing soil fertility. Thus, nitrogen-fixers are beneficial to agriculture while denitrifiers are harmful.



  1. Explain the process of vaccine preparation using bacteria.
    Vaccines are prepared using killed or weakened forms of disease-causing bacteria. Sometimes, their toxins are inactivated to form toxoids, which are used to stimulate immunity. When introduced into the body, vaccines help produce antibodies without causing the disease itself. For example, the BCG vaccine uses a weakened form of Mycobacterium bovis to protect against tuberculosis.



  1. How do serums and antitoxins work in protecting humans?
    Serums are prepared from the blood of animals or humans who have developed immunity to a disease. They contain ready-made antibodies that provide immediate protection when injected into a patient. Antitoxins are antibodies produced to neutralize specific bacterial toxins, such as tetanus antitoxin. They are used in emergency cases where quick action is required.



  1. What are toxoids? How are they useful?
    Toxoids are bacterial toxins that have been chemically inactivated but still retain their ability to stimulate the immune system. For example, diphtheria and tetanus vaccines are prepared using toxoids. They are safe for use and provide long-lasting immunity by encouraging the body to produce specific antibodies without causing disease.



  1. Explain the role of fungi in wine production.
    In wine production, yeast (Saccharomyces cerevisiae) ferments sugars present in grape juice into alcohol and carbon dioxide. This process is anaerobic and releases energy for yeast survival while producing ethanol. The type and strain of yeast, along with fermentation conditions, influence the taste, flavour, and alcohol content of wine. This method has been practiced since ancient times.



  1. How is yeast used in bread making?
    Yeast ferments the sugar present in flour dough, releasing carbon dioxide and alcohol. The carbon dioxide gets trapped in the dough, causing it to expand and rise. The alcohol evaporates during baking, leaving the bread soft, porous, and spongy. Yeast fermentation also gives bread a pleasant flavour and aroma.



  1. Give a detailed account of antibiotics produced from fungi.
    The discovery of antibiotics from fungi was revolutionary in medicine. Penicillium notatum produces penicillin, the first antibiotic used to kill bacteria by destroying their cell walls. Other fungi like Cephalosporium produce cephalosporins. These antibiotics are used to treat bacterial infections such as pneumonia, syphilis, and tonsillitis. Fungal antibiotics saved millions of lives and marked the beginning of the antibiotic era.



  1. Explain the harmful role of fungi in food spoilage.
    Fungi like Aspergillus and Rhizopus spoil food by growing on bread, fruits, and vegetables. They break down nutrients and secrete toxins, making food unfit for consumption. For example, aflatoxins produced by Aspergillus flavus contaminate grains and groundnuts, causing health hazards. Fungal spoilage not only wastes food but also poses risks to human health.



  1. What are the differences between useful and harmful fungi?
    Useful fungi include yeast for baking and brewing, Penicillium for antibiotic production, and edible mushrooms for nutrition. Harmful fungi cause diseases in plants like rusts and smuts, spoil food, and produce toxins. Thus, while useful fungi have industrial and medicinal value, harmful fungi reduce agricultural yield and cause economic losses.



  1. Explain the role of fungi in mushroom cultivation.
    Mushroom cultivation is based on growing the fruiting bodies of fungi under controlled conditions. The fungal mycelium grows in substrates like straw or compost. With proper temperature and humidity, mushrooms develop within weeks. This industry is important because mushrooms are highly nutritious and commercially valuable.



  1. How does fermentation by yeast differ in alcohol and bread production?
    In alcohol production, yeast ferments sugars under anaerobic conditions to produce ethanol, which is preserved and consumed. In bread making, fermentation also produces alcohol, but it evaporates during baking, leaving behind carbon dioxide that makes bread spongy. Thus, alcohol is the main product in wine, while carbon dioxide is the important product in bread.



  1. Describe the role of bacteria in nitrogen fixation.
    Nitrogen-fixing bacteria like Rhizobium live in root nodules of leguminous plants and convert atmospheric nitrogen into ammonia. Free-living bacteria like Azotobacter also fix nitrogen in the soil. This ammonia is later converted into nitrates, which plants use for protein synthesis. Nitrogen fixation enriches soil fertility and reduces dependence on chemical fertilizers.



  1. How do denitrifying bacteria harm soil fertility?
    Denitrifying bacteria like Pseudomonas convert nitrates in the soil back into nitrogen gas, releasing it into the atmosphere. This reduces the amount of nitrates available to plants, lowering soil fertility and agricultural productivity. Hence, denitrification is considered harmful for farming.



  1. Explain the importance of bacteria in dairy industry.
    Bacteria play a key role in the dairy industry by converting milk into products like curd, cheese, butter, and yoghurt. Lactobacillus converts lactose into lactic acid, which helps in curdling milk and giving flavour to curd. In cheese production, bacteria contribute to fermentation, texture, and aroma. Thus, bacterial action is indispensable in dairy product manufacturing.



  1. What is the importance of edible mushrooms in human diet?
    Edible mushrooms are rich in proteins, vitamins, and minerals but low in fats and calories, making them an ideal food. They also contain dietary fibres and antioxidants that promote good health. Mushrooms are used as a meat substitute for vegetarians and are highly valued in culinary practices worldwide.



  1. Describe the economic importance of fungi in baking industry.
    Fungi, especially yeast, are widely used in baking. Yeast ferments sugars in dough to release carbon dioxide, which makes bread soft and fluffy. It also produces flavouring compounds that improve taste. The baking industry depends heavily on yeast fermentation, which provides both economic and nutritional benefits.



  1. Write a detailed account of bacterial diseases in plants with examples.
    Bacteria cause several plant diseases that reduce crop yield and quality. For example, citrus canker caused by Xanthomonas citri produces lesions on leaves and fruits of lemon and orange, lowering their market value. Ring disease of potatoes caused by Clavibacter blocks xylem vessels, leading to wilting and crop loss. These diseases spread quickly through water, soil, and insects, making them difficult to control. Preventive measures include crop rotation, resistant varieties, and proper sanitation.

Give Reasons

Q.1. Give reason: Bacteria are called microscopic organisms.
 Ans. Bacteria are called microscopic organisms because they are extremely small and can only be seen under a microscope.

Q.2. Give reason: Some bacteria are called nitrogen-fixing bacteria.
 Ans. They are called nitrogen-fixing bacteria because they convert atmospheric nitrogen into ammonia and nitrates which plants can absorb.

Q.3. Give reason: Nitrifying bacteria are useful in agriculture.
 Ans. Nitrifying bacteria are useful because they convert ammonia into nitrates, which enrich soil fertility and are essential for plant growth.

Q.4. Give reason: Denitrifying bacteria are considered harmful.
 Ans. They are harmful because they convert soil nitrates back into atmospheric nitrogen, reducing soil fertility.

Q.5. Give reason: Rhizobium bacteria are found in leguminous plants.
 Ans. They live in root nodules of legumes where they fix nitrogen, benefiting the plant with nitrogen compounds.

Q.6. Give reason: Bacteria are used in vaccine preparation.
 Ans. Weakened or killed bacteria or their toxins are used to prepare vaccines that help develop immunity.

Q.7. Give reason: Toxoids are preferred in some vaccines.
 Ans. Toxoids are preferred because they are inactive toxins that safely stimulate the body to produce antibodies.

Q.8. Give reason: Antitoxins are used in emergency cases.
 Ans. They provide immediate neutralization of bacterial toxins when rapid action is needed, as in tetanus infection.

Q.9. Give reason: Serums provide immediate immunity.
 Ans. Serums contain ready-made antibodies that directly fight pathogens without waiting for the body to produce them.

Q.10. Give reason: Bacteria are important in the dairy industry.
 Ans. They help convert milk into curd, butter, cheese, and yoghurt through fermentation.

Q.11. Give reason: Curd tastes sour.
 Ans. It tastes sour because Lactobacillus bacteria ferment lactose into lactic acid, giving sourness.

Q.12. Give reason: Antibiotics are called life-saving drugs.
 Ans. They kill or inhibit disease-causing bacteria, saving humans from fatal infections.

Q.13. Give reason: Penicillin is regarded as a miracle drug.
 Ans. Penicillin, produced from Penicillium fungus, was the first antibiotic that successfully treated many bacterial diseases.

Q.14. Give reason: Yeast is widely used in baking industry.
 Ans. Yeast ferments sugar to release carbon dioxide, making dough soft, spongy, and well-risen.

Q.15. Give reason: Yeast is important in alcohol production.
 Ans. Yeast ferments sugars in fruits and grains to produce ethanol used in wine, beer, and whisky.

Q.16. Give reason: Yeast is called facultative anaerobe.
 Ans. Yeast can survive both in the presence of oxygen (respiration) and in its absence (fermentation).

Q.17. Give reason: Tea has characteristic flavour after fermentation.
 Ans. Microbial fermentation of tea leaves alters their chemical constituents, developing flavour and aroma.

Q.18. Give reason: Leather tanning sometimes uses bacteria.
 Ans. Bacterial enzymes break down unwanted proteins and fats in animal hides, making leather soft and pliable.

Q.19. Give reason: Spoiled food emits foul odour.
 Ans. Bacteria break down proteins and fats in food, releasing foul-smelling gases.

Q.20. Give reason: Spoiled food should not be eaten.
 Ans. It contains harmful bacteria and toxins that can cause food poisoning.

Q.21. Give reason: Refrigeration slows down food spoilage.
 Ans. Low temperature reduces bacterial activity and multiplication, delaying spoilage.

Q.22. Give reason: Milk is pasteurized.
 Ans. Pasteurization kills harmful bacteria while preserving useful nutrients in milk.

Q.23. Give reason: Salting helps preserve food.
 Ans. Salt draws out water from food and bacterial cells, inhibiting bacterial growth.

Q.24. Give reason: Sugar is used in preservation of jams.
 Ans. High sugar concentration reduces water availability, preventing bacterial growth.

Q.25. Give reason: Bacteria cause diseases in plants.
 Ans. Some bacteria invade plant tissues, producing toxins and blocking transport systems, leading to disease.

Q.26. Give reason: Citrus canker reduces fruit yield.
 Ans. The bacterial infection damages leaves and fruits, lowering both quality and quantity.

Q.27. Give reason: Anthrax is dangerous to cattle.
 Ans. It causes fever, swelling, and sudden death in cattle due to Bacillus anthracis.

Q.28. Give reason: Tuberculosis reduces milk production in cattle.
 Ans. Infected cattle become weak, lose weight, and produce less milk.

Q.29. Give reason: Some fungi are called edible fungi.
 Ans. Mushrooms like Agaricus are edible because they are non-toxic and highly nutritious.

Q.30. Give reason: Mushrooms are considered protein-rich food.
 Ans. They contain high-quality proteins and essential amino acids.

Q.31. Give reason: Mushrooms are low-calorie food.
 Ans. They contain very little fat and carbohydrates, making them suitable for weight control.

Q.32. Give reason: Mushrooms are considered a vegetarian substitute for meat.
 Ans. They are rich in proteins, vitamins, and minerals, similar to meat in nutritive value.

Q.33. Give reason: Bread becomes spongy when yeast is added.
 Ans. Yeast fermentation produces carbon dioxide that makes the dough porous and spongy.

Q.34. Give reason: Alcoholic beverages have different flavours.
 Ans. Different strains of yeast and varying fermentation conditions create unique flavours.

Q.35. Give reason: Fungi are used in cheese making.
 Ans. Fungi help ripen cheese and impart special flavour and texture.

Q.36. Give reason: Food grains sometimes become poisonous.
 Ans. Fungi like Aspergillus produce aflatoxins in stored grains, making them poisonous.

Q.37. Give reason: Antibiotics do not work against viral diseases.
 Ans. Antibiotics act only on bacterial cell structures, not on viruses which lack such structures.

Q.38. Give reason: Tetanus requires immediate injection of antitoxin.
 Ans. The bacterial toxin of tetanus spreads rapidly, and only antitoxin can neutralize it quickly.

Q.39. Give reason: Soil fertility is maintained by microbes.
 Ans. Nitrogen-fixing and nitrifying bacteria enrich soil by adding usable nitrogen compounds.

Q.40. Give reason: Fungi can spoil stored fruits.
 Ans. Fungi grow on moist fruits, feeding on sugars and releasing toxins.

Q.41. Give reason: Lactobacillus is useful in human diet.
 Ans. It converts milk into curd, improving digestion and nutritional value.

Q.42. Give reason: Some bacteria are used in medicine.
 Ans. They help in preparing antibiotics, vaccines, serums, and toxoids for human health.

Q.43. Give reason: Some fungi are industrially important.
 Ans. They are used in brewing, baking, wine-making, and antibiotic production.

Q.44. Give reason: Wine is considered a fermented beverage.
 Ans. It is produced by fermentation of grape juice sugars into alcohol by yeast.

Q.45. Give reason: Bacterial toxins are dangerous.
 Ans. They damage host tissues and cause severe symptoms of bacterial diseases.

Q.46. Give reason: Not all fungi are harmful.
 Ans. While some fungi cause spoilage and disease, many provide food, medicine, and industrial products.

Q.47. Give reason: Proper hygiene prevents bacterial diseases.
 Ans. Hygiene reduces bacterial entry and spread, lowering risk of infection.

Q.48. Give reason: Antibiotics should not be overused.
 Ans. Overuse leads to antibiotic resistance, making bacteria stronger and difficult to treat.

Q.49. Give reason: Food preservation is essential.
 Ans. It prevents bacterial and fungal growth, reducing food spoilage and wastage.

Q.50. Give reason: Mushrooms are commercially cultivated.
 Ans. They are nutritious, grow quickly, require little space, and provide good income.

Arrange the Words 

Case Studies

Case Study 1:
 A farmer noticed that his leguminous crops were growing very well without extra fertilizers. On examining the roots, he found small nodules.
 Q. What bacteria might be present in the nodules and what is their role?
 Ans. Rhizobium bacteria are present; they fix atmospheric nitrogen into ammonia, enriching soil fertility.

 

Case Study 2:
 A child was injected with a serum after being bitten by a dog suspected of rabies.
 Q. Why was serum given immediately instead of waiting for a vaccine?
 Ans. Serum contains ready-made antibodies providing immediate protection, whereas vaccines take time to induce immunity.

 

Case Study 3:
 A batch of bread in a bakery was soft, spongy, and had a pleasant aroma.
 Q. Which microorganism was responsible for this and how?
 Ans. Yeast (Saccharomyces cerevisiae) fermented sugars, releasing carbon dioxide (makes dough rise) and alcohol (evaporates, giving aroma).

 

Case Study 4:
 A cheese factory used Penicillium roqueforti to ripen cheese.
 Q. Why is this fungus important in cheese making?
 Ans. It imparts characteristic flavour, aroma, and texture to the cheese.

 

Case Study 5:
 A student observed some milk turning sour in the refrigerator.
 Q. Which bacteria caused this and what is the chemical change?
 Ans. Lactobacillus bacteria converted lactose into lactic acid, souring the milk.

 

Case Study 6:
 A farmer noticed yellow lesions on orange trees reducing the yield of fruits.
 Q. Identify the probable bacterial disease and its cause.
 Ans. Citrus canker caused by Xanthomonas citri.

 

Case Study 7:
 A laboratory produced penicillin to treat bacterial infections.
 Q. Which fungus produces penicillin and what is its economic importance?
 Ans. Penicillium notatum; it saves lives by treating bacterial infections, a major medical breakthrough.

 

Case Study 8:
 A stored grain stock developed mould and was found to be toxic.
 Q. Which fungus could be responsible and why is it harmful?
 Ans. Aspergillus flavus; it produces aflatoxins which are poisonous and can cause liver damage.

 

Case Study 9:
 Wine was prepared from grape juice using microorganisms.
 Q. Which microorganism is used and what process occurs?
 Ans. Yeast ferments sugars anaerobically into ethanol (alcohol) and carbon dioxide.

 

Case Study 10:
 A soil sample contained Nitrosomonas and Nitrobacter.
 Q. Explain their role in agriculture.
 Ans. Nitrosomonas converts ammonia into nitrites; Nitrobacter converts nitrites into nitrates, enriching soil fertility.

 

Case Study 11:
 A child was vaccinated with BCG at birth.
 Q. Why was this vaccine given and which disease does it prevent?
 Ans. BCG contains weakened Mycobacterium bovis and protects against tuberculosis.

 

Case Study 12:
 A farmer noticed wilting of potato plants due to bacterial infection.
 Q. Name the bacterium and how it affects the plant.
 Ans. Clavibacter; it blocks xylem vessels causing wilting and reducing crop yield.

 

Case Study 13:
 A jam manufacturer adds high sugar to preserve fruit jam.
 Q. Explain how sugar prevents spoilage.
 Ans. High sugar reduces water availability, preventing bacterial and fungal growth.

 

Case Study 14:
 A person injected with tetanus antitoxin immediately after injury.
 Q. Why was antitoxin preferred over vaccine in this case?
 Ans. Antitoxin provides immediate neutralization of bacterial toxin, whereas a vaccine takes time to induce immunity.

 

Case Study 15:
 A tea factory observed that fermented tea had improved flavour.
 Q. What role do microorganisms play in tea processing?
 Ans. Bacteria and fungi ferment tea leaves, altering chemical composition to develop flavour and aroma.

 

Case Study 16:
 A bakery uses yeast to make bread rise but the dough remained flat.
 Q. Suggest a reason for this failure.
 Ans. Yeast may be inactive due to incorrect temperature, expired yeast, or insufficient sugar to ferment.

 

Case Study 17:
 A farmer observes that crops need nitrogen fertilizer, but legumes grow well naturally.
 Q. Explain why legumes do not need extra nitrogen.
 Ans. Legumes have Rhizobium in root nodules that fix atmospheric nitrogen for plant use.

 

Case Study 18:
 Mushrooms are grown commercially under controlled conditions.
 Q. Why is controlled environment important for mushroom cultivation?
 Ans. Temperature, humidity, and substrate must be controlled for optimum growth and yield of mushrooms.

 

Case Study 19:
 A bakery prepares alcoholic beverages using yeast.
 Q. What is the process and what is the end product?
 Ans. Yeast ferments sugars anaerobically into ethanol; end product is alcohol (wine, beer, etc.).

 

Case Study 20:
 Stored vegetables develop foul smell after a few days.
 Q. Explain the cause and how it can be prevented.
 Ans. Bacteria and fungi decompose vegetables, producing foul-smelling gases; prevention includes refrigeration, salting, or canning.

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